HigherPurity™ RNA Total (All sizes) Isolation Kit is ideal for an efficient, high quality and easy isolation and purification of Total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi or plants. The kit purifies all size of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). Purification is based on spin column chromatography.

Features:

• High quality of Total RNA obtained.
• Efficient: streamlined protocol isolation of small RNA species using a 2 column process.
• Convenient: proven performance in all sizes of RNA, including all small RNA species (<200 nt) including miRNA, siRNA, tRNA and 5S rRNA.
• Safe: avoids the use of harmful chemicals as phenol or chloroform.
• Easy and fast protocol: results in 30-45 minutes.
• Pure RNA: ready-to-use in all downstream applications.

Includes for 50 reactions:

• 22 mL RNA Buffer Lysis
• 20 mL RNA Wash Buffer
• 6 mL RNA Elution Buffer
• 50 microRNA Spin Columns
• 100 Collection tubes

Applications:

• real-time RT-PCR.
• RT-PCR.
• Northern blotting.
• Primer extension.
• RNase protection.
• Expression array assays.
• microRNA Cloning.