HigherPurity™ Stool DNA Isolation Kit provides a reliable, easy and convenient technique to isolate high quality DNA from fresh or frozen stool samples. The kit uses HigherPurity™ breakthrough technology based in DNA ability to bind silica in the presence of high concentrations of chaotropic salts as guanidinium thiocyanate.

Fecal samples are rapidly and efficiently lysed by bead beating. The sample DNA is then bound to the surface of a Spin Filter membrane and washed and the bound DNA is then desorbed from the surface of the Spin Filter column. The inhibitors of the downstream PCR will be removed by utilizing the DNA binding column and the buffers system included in the kit.

Features:

• High purity: eliminates humic acid, polysaccharides, phenol compounds and enzyme inhibitors from stool samples.
• Efficient: fecal samples are lysed by bead beating.
• Time-saving protocol: rapid isolation of ready-to-use DNA within 54 minutes with minimal handling steps.
• Safe and convenient: avoids phenol/chloroform extraction.
• Sample Size: 50~200 mg of fresh or frozen stool sample.
• Extremely easy procedure: to isolate high quality DNA from fresh or frozen stool samples.

Includes for 50 reactions:

• 50 CleanEasy™ MiniSpin Columns
• 100 Collection tubes (2 mL)
• 50 Dry bead tube
• 50 microcentrifuge tube (1.5 mL)
• 12 g Glass Beads
• 20 mL Lysis Solution 1 (LS1)
• 15 mL Buffer A
• 15 mL Inhibitor Removal Buffer (IR-Buffer)
• 20 mL Buffer B
• 20 mL WB1 Buffer
• 20 mL EB Buffer
• 15 mg Proteinase K

Purified DNA suitable for all common Molecular Biology applications, such as:

• Digestion with restriction enzymes.
• Automated sequencing.
• PCR template.
• Southern Blots.