AgaPure™ Agarose LM is agarose chemically modified in which hydroxyethyl groups have been introduced into the agarose molecule. This substitution causes the agarose to gel at 30 ºC and to melt at 65 ºC. The lower gelling temperature ensures the agarose will be in a liquid state at a temperature range where “in-gel” manipulations can be performed without prior extraction of the DNA or RNA from the gel slice. Also, the properties of low melt agarose promote quick and thorough digestion of gel slices during extraction procedures. Agarose LM has low EEO (electroendosmosis, ≤0.1), allowing for sharp DNA banding and high resolution.

Features:

• Highest quality: free of DNase, RNase, electrically neutral and Biologically inert.
• Exceptional clarity: improves visualization, even at high concentrations.
• Low background: low absorption of staining agents.

Specifications:

• Appearance: White, fine, homogeneous, free flowing.
• Molecular Biology Grade.
• Melting Point (1.5%) ≤ 65.5 ºC
• Gel strength (1.5%): 3130 g/cm2
• Gelling temperature (1.5%): 24-28ºC
• Sulphate: 0.076%
• EEO(pH 8.4, Wieme Method) ≤  0.12
• Ash: 0.37 %

Includes for 50 g:

• 50 g AgaPure™Agarose LM (Low melting point)

Applications:

•  Conventional and preparative electrophoresis of nucleic acids in a 100–25.000 bp range
• Recovery of high quality RNA and DNA after electrophoretic separation
• Direct enzymatic manipulations in the dissolved agarose gel