MagBeads™ Bacteria G (-) Genomic DNA Isolation

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For a consistent, easy and rapid high quality DNA isolation from Gram-negative bacteria.

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Category: Investigación.
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MagBeads™ Bacteria G (-) Genomic DNA Isolation Kit provides a consistent, easy-to use and rapid method to isolate high quality DNA from Gram negative bacteria. The kit uses MagBeads™ breakthrough technology for extraction based on paramagnetic beads.

It has been optimized specifically for isolating bacterial DNA from cell pellets after culturing. The extraction process includes an initial cell-wall lysis step with the appropriate Buffer to ensure efficient cell lysis and DNA release from the cell, DNA binding to the surface of the magnetic beads, washing and elution.

Features:

  • High quality: 100% chloroform and phenol free.
  • Safe: avoids the use of dangerous and polluting chaotropic salts.
  • Harmless: ideal for the downstream applications.
  • Time-saving protocol: avoids the centrifugation step in the DNA purification process.
  • Cost avoidance: saves on equipment costs and increases portability of the technique.

Includes for 50 reactions:

  • 0.5 mL MagBeads™ solution
  • 15 mL Lysis Buffer solution 1
  • 5 mL Lysis Buffer solution 2
  • 10 mL WB1 Buffer
  • 5 mL EB Buffer
  • 500 µL RNAse A

All Molecular Biology applications, such as:

  • Digestion with restriction enzymes.
  • Automated sequencing.
  • PCR template.
  • Southern Blots.
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