HigherPurity™ Food DNA Purification Kit has been optimized for an efficient and fast purification of total DNA from food samples (raw material and processed food).

After the samples have been homogenized, the DNA can be extracted with the extraction buffer, lysis mixtures should be cleared by centrifugation or filtration in order to remove contaminants and residual cellular debris. The clear supernatant is then mixed with the Binding Buffer, Proteinase K and isopropanol to create conditions for optimal binding to the silica membrane column. After washing with two different buffers for efficient removal of potential PCR inhibitors, DNA can be eluted in low salt buffer or water, and is ready-to-use in subsequent reactions.

Features:

• High quality: of DNA obtained, ready-to-use in all Molecular Biology applications, due its complete removal of contaminants and inhibitors.
• Sample size: up to 200 mg.
• Fast and easy protocol.
• Versatile: isolate high quality DNA from a wide range of food materials.
• Powerful: even low amounts of partially degrade DNA can be purified from complex matrices.
• Convenient Silica membrane technology.

Includes for 50 reactions:

• 50 Minispin columns
• 100 Collection tubes (2 mL)
• 65 mL Lysis Solution A (LSA)
• 35 mL Lysis Solution B (LSB)
• 16.5 mL WB1 Buffer
• 10 mL EB Buffer
• 22 mg Proteinase K
• 10 mL WB2 Buffer

Applications:

• DNA from complex matrices, processed food, soya, chocolate, cereals, meat, animal feed.
• Detection of genetically modified material in food products.
• DNA suitable for PCR, real-time PCR, Southern blotting, enzymatic reactions.
• Detection of specific DNA in animal feed.