WideUSE™ Plasmid Midi/Maxiprep Kit offers a fast, simple and efficient method for the routinary isolation of high quality Plasmid preparations in Midiprep/Maxiprep format.

It introduces the Lysis control reagent (LCR) a color indicator which provides visual identification of optimum buffer mixing. This prevents common handling errors that lead to inefficient cell lysis and incomplete precipitation of SDS, genomic DNA and cell debris. This makes ideal for use by researchers who have not much experience with plasmid preparation as well as experienced scientists who want to be assured of maximum product yield.

Features:

• High quality: of Plasmid DNA obtained, ready-to-use in all Molecular Biology applications. Achieve transfection grade plasmid purity.
• Fast: results in few minutes.
• Safe: avoids phenol-chloroform extraction.
• Complete solution: includes specialized filters to optional remove cellular debris from lysates.
• Midi and Maxi format.
• Technology: based on familiar anion-exchange columns.

Includes for Midiprep format (ref. AN0168):

• 110 mL Resuspension Solution
• 150 mL Lysis Solution (BLY)
• 110 mL Neutralization Solution
• 130 mL Equilibration Buffer
• 360 mL Wash Buffer (WB1)
• 220 mL Elution Buffer (EB)
• 1,500 µL Lysis control reagent (LCR)
• 25 mL TE Buffer
• 25 Plasmid Midi / Maxi Columns
• 200 µL RNase (50 mg/mL)
• 25 Folder Filters

Includes for Maxiprep format (ref. AN0169):

• 110 mL Resuspension Solution
• 150 mL Lysis Solution (BLY)
• 110 mL Neutralization Solution
• 130 mL Equilibration Buffer
• 360 mL Wash Buffer (WB1)
• 130 mL Elution Buffer (EB)
• 1,500 µL Lysis control reagent (LCR)
• 25 mL TE Buffer
• 10 Plasmid Midi / Maxi Columns
• 200 µL RNase (50 mg/mL)
• 10 Folder Filters

All Molecular Biology applications, such as:

• Digestion with restriction enzymes.
• Automated sequencing.
• PCR template.
• Bacterial transformation.
• Transfection.