HigherPurity™ Soil DNA Isolation Kit provides a high quality, convenient and easy-to-use method technique to isolate high quality DNA from soil samples. The kit uses HigherPurity™ breakthrough technology based in DNA ability to bind silica in the presence of high concentrations of chaotropic salts as guanidinium thiocyanate.

Extractions are rapidly and efficiently lysed by bead beating. The sample DNA is then bound to the surface of a silica membrane that is inside the CleanEasy™ Spin Columns and washed and the bound DNA is then desorbed from the surface of the membrane. The inhibitors of the downstream PCR will be removed with the buffers system included in the kit.

Features:

• High quality DNA isolated from soil samples.
• Time-Saving: rapid isolation of ready-to-use DNA within 58 minutes.
• Easy, safe and convenient: avoids phenol/chloroform extraction.
• High purity: eliminate humic acid, polysaccharides, phenol compounds and enzyme inhibitor from stool samples.
• Sample Size: mini Kit Prep: 0.2~ 1 g of soil sample.
• Efficient: soil samples are lysed by bead beating.

Includes for 50 reactions:

• 50 CleanEasy™ MiniSpin Columns
• 100 Collection tubes (2 mL)
• 50 Dry bead tube
• 12 g Glass Bead
• 40 mL Lysis Solution 1 (LS1)
• 20 mL WB1 Buffer
• 35 mL EB Buffer
• 15 mg Proteinase K
• 15 mL Inhibitor Removal Buffer (IR-Buffer)
• 50 microcentrifuge tube (1.5 mL)
• 15 mL Buffer A
• 25 mL Buffer

Purified DNA suitable for all common Molecular Biology applications, such as:

• Digestion with restriction enzymes.
• Automated sequencing.
• PCR template.
• Southern Blots.