pColiExpress™ IV Glue Enzyme Kit is a highly efficient, versatile and fast system of DNA cloning vectors for protein expression in E. coli. All family vectors are based in a breakthrough technology which allows cloning efficiently DNA fragments and the fast production of a large quantity of any desired protein.

It contains all elements required for the expression of large quantities of any desired protein by the T7 RNA Polymerase Inducible System, and a His6 tag at COOH end of the protein that allows the purification. It contains at the NH2 end the Cmyc tag followed by a 3C from human Rhinovirus (HRV) Protease cleavage site, too. The vector has also an f1 origin of replication, an Ampicillin resistance cassette, and the pBR322 origin of replication.

Specifications:

• T7 Promoter: 4605-4621
• LacO: 4627-4645
• T7 transcription start: 4621
• Cmyc: 4697-4735
• Prescission cleavage site: 4736-4760
• His6 Tag: 4768-4785
• T7 terminator: 4807-4855
• LacIq: 96-1175
• Ori pBR322: 2970-2356
• AMP R (Ampicillin resistance gene): 3133-3990
• f1 Origin: 4562-4115

Features:

• Ready-to-use vectors.
• Highly efficient cloning system.
• Special design that allows the cell to house larger numbers of copies than other plasmids with ori pBR322.
• Linearized vector: ready for ligation with your PCR amplified with the recommended primers.
• Low background: < 1%.
• Time-saving protocol: avoids any step required after PCR.
• Higher protein expression than other suppliers.
• Cost avoidance: avoids the use of expensive phosphorylated primers.
• Versatility: cloning of PCR fragments amplified with any type of DNA Polymerase.

Includes for 20rxn:

• 20 µL pColiExpress™ (50 ng/µL)
• 50 µL Glue Enzyme Buffer (10x)
• 40 µL Glue Enzyme (10 UI/µL)
• 10 µL Insert Control DNA (30 ng/µL)
• 5 µL pColiExpress™ Control (50 ng/µL)

Applications:

• Cloning of PCR fragments for subsequent expression of proteins in E. coli.
• Cloning of PCR fragments with Glue Enzyme.
• Expression of proteins under the control of the T7 promoter.
• Protein expression in BL21 (DE3) or BL21 (DE3) (pLys).