For a highly efficient and versatile protein Expression in E. coli from PCR fragment cloned
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pColiExpress™ IV Glue Enzyme Kit is a highly efficient, versatile and fast system of DNA cloning vectors for protein expression in E. coli. All family vectors are based in a breakthrough technology which allows cloning efficiently DNA fragments and the fast production of a large quantity of any desired protein.
It contains all elements required for the expression of large quantities of any desired protein by the T7 RNA Polymerase Inducible System, and a His6 tag at COOH end of the protein that allows the purification. It contains at the NH2 end the Cmyc tag followed by a 3C from human Rhinovirus (HRV) Protease cleavage site, too. The vector has also an f1 origin of replication, an Ampicillin resistance cassette, and the pBR322 origin of replication.
Includes for 20rxn: