pColiExpress™ IV Glue Enzyme Kit

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For a highly efficient and versatile protein Expression in E. coli from PCR fragment cloned

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pColiExpress™ IV Glue Enzyme Kit is a highly efficient, versatile and fast system of DNA cloning vectors for protein expression in E. coli. All family vectors are based in a breakthrough technology which allows cloning efficiently DNA fragments and the fast production of a large quantity of any desired protein.

It contains all elements required for the expression of large quantities of any desired protein by the T7 RNA Polymerase Inducible System, and a His6 tag at COOH end of the protein that allows the purification. It contains at the NH2 end the Cmyc tag followed by a 3C from human Rhinovirus (HRV) Protease cleavage site, too. The vector has also an f1 origin of replication, an Ampicillin resistance cassette, and the pBR322 origin of replication.


  • T7 Promoter: 4605-4621
  • LacO: 4627-4645
  • T7 transcription start: 4621
  • Cmyc: 4697-4735
  • Prescission cleavage site: 4736-4760
  • His6 Tag: 4768-4785
  • T7 terminator: 4807-4855
  • LacIq: 96-1175
  • Ori pBR322: 2970-2356
  • AMP R (Ampicillin resistance gene): 3133-3990
  • f1 Origin: 4562-4115


  • Ready-to-use vectors.
  • Highly efficient cloning system.
  • Special design that allows the cell to house larger numbers of copies than other plasmids with ori pBR322.
  • Linearized vector: ready for ligation with your PCR amplified with the recommended primers.
  • Low background: < 1%.
  • Time-saving protocol: avoids any step required after PCR.
  • Higher protein expression than other suppliers.
  • Cost avoidance: avoids the use of expensive phosphorylated primers.
  • Versatility: cloning of PCR fragments amplified with any type of DNA Polymerase.

Includes for 20rxn:

  • 20 µL pColiExpress™ (50 ng/µL)
  • 50 µL Glue Enzyme Buffer (10x)
  • 40 µL Glue Enzyme (10 UI/µL)
  • 10 µL Insert Control DNA (30 ng/µL)
  • 5 µL pColiExpress™ Control (50 ng/µL)


  • Cloning of PCR fragments for subsequent expression of proteins in E. coli.
  • Cloning of PCR fragments with Glue Enzyme.
  • Expression of proteins under the control of the T7 promoter.
  • Protein expression in BL21 (DE3) or BL21 (DE3) (pLys).
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