pColiExpress™ III Glue Enzyme Kit

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For a highly efficient and versatile protein Expression in E. coli from PCR fragment cloned

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pColiExpress™ III Glue Enzyme Kit is a highly efficient, versatile and fast system of DNA cloning vectors for protein expression in E. coli. All family vectors are based in a breakthrough technology which allows cloning efficiently DNA fragments and the fast production of a large quantity of any desired protein.

It contains all elements required for periplasmic expression of any desired protein by OMPA Periplasmatic Signal peptide and T7 RNA Polymerase Inducible System. It includes a His6 tag at COOH end of the protein that allows the purification. The vector also has an f1 origin of replication, an Ampicillin resistance cassette, and the pBR322 origin of replication.


  • T7 Promoter: 4605-4621
  • LacO: 4627-4645
  • T7 transcription start: 4621
  • Signal Peptide OmpA: 4696-4777
  • His6 Tag: 4786-4803
  • T7 terminator: 4825-4872
  • LacIq: 96-1175
  • Ori pBR322: 2970-2356
  • AMP R (Ampicillin resistance gene): 3133-3990
  • f1 Origin: 4562-4115


  • Ready-to-use vectors.
  • Highly efficient cloning system.
  • Special design that allows the cell to house larger numbers of copies than other plasmids with ori pBR322.
  • Linearized vector: ready for ligation with your PCR amplified with the recommended primers.
  • Low background: < 1%.
  • Time-saving protocol: avoids any step required after PCR.
  • Higher protein expression than other suppliers.
  • Cost avoidance: avoids the use of expensive phosphorylated primers.
  • Versatility: cloning of PCR fragments amplified with any type of DNA Polymerase.

Includes for 20rxn:

  • 20 µL pColiExpress™ (50 ng/µL)
  • 50 µL Glue Enzyme Buffer (10x)
  • 40 µL Glue Enzyme (10 UI/µL)
  • 10 µL Insert Control DNA (30 ng/µL)
  • 5 µL pColiExpress™ Control (50 ng/µL)


  • Cloning of PCR fragments for subsequent expression of proteins in E. coli.
  • Cloning of PCR fragments with Glue Enzyme.
  • Expression of proteins under the control of the T7 promoter.
  • Protein expression in BL21 (DE3) or BL21 (DE3) (pLys).
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