FastCONTROL™ p2RVa-LacZ/ eGFP – Retroviral Dual Reporter Plasmid

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To fast, convenient and flexible target cell Transfection Control

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FastCONTROL™ Dual Reporter Plasmid Controls are fast, convenient and flexible target cell transfection control ideal for high co-expression of two reporter genes drive for ubiquitous, strong and constitutive promoters. FastCONTROL™ p2RVa-LacZ/ eGFP Dual Reporter is a Retroviral vector that contains elongation factor 1 alpha promoter (PEF1α) to drive the expression of both beta galactosidase (LacZ gene) and green fluorescent protein from Aequoria victoria (eGFP). This is a self-inactivating (SIN) Retroviral vector that lack viral promoter and enhancer activity in their 3′ long terminal repeat (LTR).

  • Fast: available combination of 2 cell location markers in one vector.
  • Convenient: available in Lentiviral, Retroviral or non-viral vectors, for immediate in vivo and in vitro expression.
  • Compatible with transient or stable transfections.
  • Flexible: available with different mammalian resistance markers and Dual Reporter genes combination for different cell location.

Includes for 100 assays:

  • 15 µL FastCONTROL™ Dual Reporter Plasmid Control (1 µg/µL)

In Plus version includes for 100 assays:

  • 15 µL FastCONTROL™ Dual Reporter Plasmid Control (1 µg/µL)
  • 0.2 mL CANFAST™ Transfection Reagent (1 mg/mL)
  • 15 μL pASSEMBLE™ Viral Packaging Vector (1 μg/ μL)æ

æ ecotropic (tropism for rat or mouse cells) or amphotropic (tropism for most mammalian cells; hamster are not included).

Applications

  • Control for assessing the efficiency of transfection in mammalian cells.
  • Targering of different cell locations.
  • Obtention of cell lines with reporter proteins.
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