PickMutant™ is a reliable, robust and highly efficient PCR-based mutagenesis kit. Extremely easy-to-use, the kit allows creating single or multiple point mutations, deletions or insertions using a rapid and easy protocol. All these mutation could be obtained by PCR using a FastPANGEA™ High Fidelity DNA Polymerase and well-designed mutagenesis primers. The assembled mutagenic PCR fragments is cloned into pSpark® cloning vector, specially designed to clone blunt PCR fragments with high efficiency or into other vector designing, in this case, an additional specific vector primer pair.

For 15 reactions.

• Highly Effective point mutations (single or multiple), deletions or insertions.
• Easy and fast protocol: it takes less than 3 hours in one step procedure.
• Cost avoidance: compatible with any bacterial strains or primers.
• Versatile: compatible with any cloning vector.
• Efficient: includes highly efficient pSpark® to clone blunted fragments.
• Robust: simultaneous assemble and clone of PCR fragments.

Includes for 15 rxn:

• 150 μL Proofreading DNA Polymerase MasterMix (2x)
• 300 U Glue enzyme (10 U/μL)
• 40 μL Glue enzyme Buffer (10x)
• 5 μL Insert Control DNA
• 15 μL pSpark® I (20 ng/μL)

Applications:

• Site-directed Mutagenesis.
• Study protein function.
• Identify enzyme active sites.
• Design new proteins.