PickMutant™ Site-directed Mutagenesis Kit - 15 Reactions

MT001

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For a reliable, robust and highly efficient Site-directed Mutagenesis based in PCR

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PickMutant™ is a reliable, robust and highly efficient PCR-based mutagenesis kit. Extremely easy-to-use, the kit allows creating single or multiple point mutations, deletions or insertions using a rapid and easy protocol. All these mutation could be obtained by PCR using a FastPANGEA™ High Fidelity DNA Polymerase and well-designed mutagenesis primers. The assembled mutagenic PCR fragments is cloned into pSpark® cloning vector, specially designed to clone blunt PCR fragments with high efficiency or into other vector designing, in this case, an additional specific vector primer pair.

For 15 reactions.

  • Highly Effective point mutations (single or multiple), deletions or insertions.
  • Easy and fast protocol: it takes less than 3 hours in one step procedure.
  • Cost avoidance: compatible with any bacterial strains or primers.
  • Versatile: compatible with any cloning vector.
  • Efficient: includes highly efficient pSpark® to clone blunted fragments.
  • Robust: simultaneous assemble and clone of PCR fragments.

Includes for 15 rxn:

  • 150 μL Proofreading DNA Polymerase MasterMix (2x)
  • 300 U Glue enzyme (10 U/μL)
  • 40 μL Glue enzyme Buffer (10x)
  • 5 μL Insert Control DNA
  • 15 μL pSpark® I (20 ng/μL)

Applications:

  • Site-directed Mutagenesis.
  • Study protein function.
  • Identify enzyme active sites.
  • Design new proteins.
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