X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside) is a chromogenic substrate for beta-galactosidase.

β-Galactosidase cleaves the substrate and releases colorless galactose and 5-Bromo-4-chloro-3-indoxyl (X). The indoxyl will be oxidized to the insoluble 5,5′-dibromo-4,4′-dichloro-indigo, forming an intense blue precipitate.

• CAS Number: 7240-90-6
• Chemical Formula: C₁₄H₁₅BrClNO₆
• Molecular Weight: 408.63
• Assay (HPLC) > 98% w/w
• Purity (HPLC): > 99%
• Purity (TLC): single spot
• Water content (Karl Fischer): <1%
• Identity (IR): conforms to structure
• Solubility (5% w/v, DMF): soluble
• Reliable: stringent quality control standards to guarantee lot-to-lot consistency.
• High Quality.
• Powder format.

Includes for 5 g:

• 5 g X-Gal

Applications:

• Blue/white screening.
• Gene expression detection of β-galactosidase reporter.
• Detection of β-galactosidase activity in immunological and histochemical applications.