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5-Bromo-4-chloro-3-indolyl beta-D-Galactopyranoside.

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X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside) is a chromogenic substrate for beta-galactosidase.

β-Galactosidase cleaves the substrate and releases colorless galactose and 5-Bromo-4-chloro-3-indoxyl (X). The indoxyl will be oxidized to the insoluble 5,5′-dibromo-4,4′-dichloro-indigo, forming an intense blue precipitate.

  • CAS Number: 7240-90-6
  • Chemical Formula: C14H15BrClNO6
  • Molecular Weight: 408.63
  • Assay (HPLC) > 98% w/w
  • Purity (HPLC): > 99%
  • Purity (TLC): single spot
  • Water content (Karl Fischer): <1%
  • Identity (IR): conforms to structure
  • Solubility (5% w/v, DMF): soluble
  • Reliable: stringent quality control standards to guarantee lot-to-lot consistency.
  • High Quality.
  • Powder format.

Includes for 5 g:

  • 5 g X-Gal


  • Blue/white screening.
  • Gene expression detection of β-galactosidase reporter.
  • Detection of β-galactosidase activity in immunological and histochemical applications.
Path: > > X-Gal

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