CVX5α™ Chemically Competent Cells is a versatile and cost-effective solution for routine subcloning procedures or any application where the starting DNA is not limiting. It is calcium chloride-treated to facilitate attachment of the plasmid DNA to the competent cell membrane.

pCVX5α™ Genotype: F – , gyrA96, recA1, endA1, thi1, hsdR17 (rK – mK +), deoR, supE44, ∆ (lacZYA-argF) U169 φ80lacZ∆M15 (similar to DH5™ ).

• Versatile: proven performance for high-efficiency transformation in a wide variety of applications.
• Convenient: ideal for routine.
• Compatible: with blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal.
• Cost avoidance: dry ice free of charge.

Includes for 40 rxn:

• 2,000 µL CVX5α™ (1 x 107 CFU/µg)
• 10 µL pUC18 Transformation Control Plasmid (10 ng/µL)
• 50 mL SOC Medium
• Dry ice

Applications:

• Routine cloning and subcloning of genes into plasmid vectors.