pSpark® Universal is a highly efficient, accurate and easy-to-use DNA cloning kit ideal for a broad range of PCR fragments cloning applications. There is a range of DNA polymerases available that do not generate PCR products with identical ends: proofreading DNA polymerases leave blunt ends while blends of polymerases and non-proofreading DNA polymerases leaves 3´A overhangs. Therefore, it is necessary to employ different vectors to clone both kinds of PCR fragments.

pSpark® Universal DNA cloning kit has been designed to save time, looking for a kit for several cloning scenarios. It is mainly composed of two cloning vectors which allow blunt or TA DNA cloning. For blunt DNA cloning and TA DNA cloning, pSpark® II DNA cloning vector and pSpark® TA DNA cloning vector, respectively, are included.

• Compatible with Blunt and TA DNA cloning: it is composed by pSpark® II and pSpark® TA DNA Cloning vectors.
• Convenient: ideal for a broad range of PCR fragments cloning applications.
• Versatile: compatible with any DNA polymerase.

Includes for 20 rxn:

• 20 µL pSpark® II (20 ng/µL)
• 20 µL pSpark® TA (50 ng/µL)
• 20 µL T4 DNA Ligase (5U/Weiss)
• 200 µL T4 DNA Ligase Buffer (5x)
• 150 µL PEG 6000 (10x)
• 1 kb Insert Control (20 ng/µL)
• Insert Control TA (30 ng/µL)

Applications:

• Cloning of high fidelity PCR amplified products into pSpark® II Blunt DNA cloning vector.
• Cloning of non-proofreading PCR fragments into pSpark® TA DNA Cloning vector.
• Production of ssDNA.
• In vitro transcription from T7/SP6 dual-opposed promoters.